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Chapter 1: Principle
Chapter 2: Kit Contents
Chapter 3: Reagent Preparation
Chapter 4: Weighing of Samples, Addition of Buffer plus Amylase & AMG
and Incubation at 37ºC for 16 hours
Chapter 5: Addition of Tris Base and Inactivation of Amylase & AMG
Chapter 6: Incubation with Protease
Chapter 7A: Determination of HMWDF & LMWSDF
pH Adjustment & Addition of Glycerol & Ethanol
(i): Filtration & Recovery of HMWDF & LMWSDF
(ii): Ash Determination
(iii): Protein Determination
(iv): Evaporation of Aqueous Ethanol & Recovery of LMWSDF
(v): Desalting of Samples with Ion Exchange Resin
(vi): Liquid Chromatography of Desalted Samples
Chapter 7B: Determination of IDF, HMWSDF & LMWSDF
(i): Filtration and Recovery of IDF, HMWSDF & LMWSDF
Chapter 8: Calculation of Dietary Fibre Content
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