High purity Sucrase (from yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Sucrase (E-SUCR) and Fructanase (E-FRMXLQ or E-FRMXPD) are used in the measurement of fructan in foods according to Chinese GB Standard 5009.255-2016 and in the AOAC method 2016.06.
Content: 300 Units (on sucrose)
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: > 1 year under recommended storage conditions
Enzyme Activity: Sucrase/Invertase
EC Number: 3.2.1.20
CAZy Family: GH13
CAS Number: 9001-42-7
Synonyms: alpha-glucosidase; alpha-D-glucoside glucohydrolase
Source: Yeast
Molecular Weight: 62,000
Expression: Purified from Yeast
Specificity: Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose.
Specific Activity: ~ 20 U/mg (30oC, pH 6.8 on sucrose)
Unit Definition: One Unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 30oC.
Temperature Optima: 30oC
pH Optima: 6.8
Application examples: Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries.
Method recognition: AOAC Method 2016.06 and GB Standard 5009.255-2016
用于研究、生化酶测定和体外诊断分析的高纯度蔗糖酶（来自酵母）。根据中国GB标准5009.255-2016和AOAC方法2016.06，蔗糖酶（E-SUCR）和果聚糖酶（E-FRMXLQ或E-FRMXPD）用于食品中果聚糖的测量。
含量：300单位（蔗糖）
运输温度：环境温度
储存温度：低于-10oC
配方：以冻干粉末形式提供
物理形态：粉末
稳定性：在推荐的储存条件下>1年
酶活性：蔗糖酶/转化酶
EC编号：3.2.1.20
CAZy家族：GH13
CAS编号：9001-42-7
同义词：α-葡萄糖苷酶；α-D-葡糖苷葡糖水解酶
来源：酵母
分子量：62000
表达：从酵母中纯化
特异性：末端非还原性（1,4）连接的α-D-葡萄糖残基水解，释放D-葡萄糖。
比活性：~20 U/mg（30℃，蔗糖pH 6.8）
单位定义：蔗糖酶活性的一个单位定义为在30℃、pH 6.8的马来酸钠缓冲液（100 mM）中，每分钟从蔗糖（10 mM）释放1µmol葡萄糖所需的酶量。
最佳温度：30oC
pH Optima:6.8
应用实例：在谷物、食品和饲料、发酵和饮料行业的各种分析程序中去除蔗糖的应用。
方法识别：AOAC方法2016.06和GB标准5009.255-2016
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