用于测定内切木聚糖酶（内切-1,4-β-木聚糖酶）的XylX6测定试剂含有两种成分；1）4,6-O-（3-酮丁基）-4-硝基苯基-β-D-45-葡萄糖基-木五糖苷和2）β-木糖苷酶。酮阻断基团可防止β-木糖苷酶或其他外显作用糖苷酶对XylX6底物的任何水解作用。与内切木聚糖酶一起孵育会产生一种非封闭的比色低聚糖，该低聚糖可被辅助的β-木糖苷酶快速水解。因此，4-硝基苯酚的形成速率与木聚糖酶水解XylX6直接相关。反应终止，加入Tris缓冲溶液（pH=10.0）后出现酚盐颜色。

需要测量其他酶的活性吗？查看我们完整的酶活性测定试剂盒列表。

请注意，使用XylX6测定法获得的吸光度与天然底物小麦阿拉伯木聚糖和山毛榉木聚糖上内切木聚糖酶活性的标准曲线在“文件”选项卡下的“支持信息”文件中提供。

优势

非常划算

所有试剂稳定4年以上

对内切-1,4-β-木聚糖酶完全特异

一般适用且高度敏感

简单的格式。非常适合自动化

出色的再现性

标准包括

内容：（K-XylX6-2V）200次测定（手动）/400次测定（自动分析仪）

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：见单个组件标签

稳定性：在推荐的储存条件下>2年

分析物：内切-1,4-β-木聚糖酶

分析格式：分光光度计、自动分析仪

检测方法：吸光度

波长（nm）：400，

405

信号响应：增加

检测限：5.3 x 10-4 U/mL

再现性（%）：~3%

总检测时间：10分钟

应用实例：发酵液、工业酶制剂、动物饲料、生物燃料研究、大麦芽分析。

方法识别：新方法

 

The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-45-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Need to measure other enzyme activities? View our complete list of assay kits for measurement of enzyme activity.

Note that standard curves relating the absorbance obtained using the XylX6 assay to endo-xylanase activity on the native substrates, wheat arabinoxylan and beechwood xylan, are provided in the Supporting Information file under the Documents tab.

Advantages

Very cost effective 

All reagents stable for > 4 years 

Completely specific for endo-1,4-β-xylanase 

Generally applicable and highly sensitive 

Simple format. Well suited to automation 

Excellent reproducibility 

Standard included

Content: (K-XylX6-2V) 200 assays (manual) / 400 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: endo-1,4-β-Xylanase

Assay Format: Spectrophotometer, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 400,

405  

Signal Response: Increase

Limit of Detection: 5.3 x 10-4 U/mL

Reproducibility (%): ~ 3%

Total Assay Time: 10 min

Application examples: Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley malt analysis.