K-RAPRS_DATA中文版说明书.pdf

The Resistant Starch Assay Kit (Rapid) method is suitable for the analysis of resistant starch in pure starch, cereal and legume seeds and food samples.

This method is an update of the method of McCleary et al1 (AOAC Method 2002.02, AACC Method 32-40.01) employing incubation conditions similar to those used in AOAC Method 2017.16 for dietary fiber. The enzyme mixture employed [pancreatic α-amylase (PAA) and amyloglucosidase (AMG)] are those used by Englyst et al.2 except that both enzymes have been purified, standardised and stabilised. Digestion is performed using saturating levels of PAA and AMG with stirring at pH 6 and 37oC for 4 h, to simulate in vivo conditions in the human small intestine. Recent studies on the hydrolysis of “newer” resistant starch materials such as phosphate crosslinked starch (RS4) indicated that these incubation conditions are an essential requirement to obtain meaningful physiologically relevant values for RS.

The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to service the definition of DF announced by Codex Alimentarius in 2009.

1. McCleary, B. V., McNally, M. & Rossiter, P. (2002).  Measurement of Resistant Starch by Enzymic Digestion in Starch and Selected Plant Materials - Collaborative Study.  J. AOAC Int., 85, 1103-1111.

2. Englyst, H. N., Kingman, S. M. & Cummings, J. H. (1992).  Classification and measurement of nutritionally important starch fractions.  Eur. J. Clin. Nutr., 46 (Suppl. 2), S33-S50.

See more of our starch assay kits.

Content: 100 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Resistant Starch

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 4 to 100 mg of D-glucose per assay

Limit of Detection: 0.036 g/100 g

Reaction Time (min): ~ 5 h

Application examples: Plant materials, starch samples and other materials.

Method recognition: Novel method

抗性淀粉测定试剂盒（快速）法适用于分析纯淀粉、谷物和豆类种子以及食品样品中的抗性淀粉。

该方法是McCleary等人1的方法（AOAC方法2002.02，AACC方法32-40.01）的更新，使用与AOAC方法2017.16中使用的膳食纤维培养条件相似的培养条件。使用的酶混合物[胰腺α-淀粉酶（PAA）和淀粉葡萄糖苷酶（AMG）]是Englyst等人2使用的酶，除了这两种酶都经过纯化、标准化和稳定化。使用饱和水平的PAA和AMG在pH 6和37℃下搅拌4小时进行消化，以模拟人体小肠的体内条件。最近对“新”抗性淀粉材料（如磷酸交联淀粉（RS4））水解的研究表明，这些培养条件是获得RS有意义的生理相关值的必要条件。

孵育条件与AOAC方法2017.16中使用的条件相似，AOAC方法是一种新的快速综合测定总膳食纤维的方法（Megazyme方法K-RINTDF）。该方法以生理学为基础，旨在为食品法典委员会2009年宣布的DF定义提供服务。

1.McCleary，B.V.、McNally，M.和Rossiter，P.（2002）。用酶消化法测定淀粉和选定植物材料中的抗性淀粉——合作研究。《AOAC国际期刊》，851103-1111。

2.Englyst，H.N.、Kingman，S.M.和Cummings，J.H.（1992）。营养重要淀粉组分的分类和测定。欧洲临床杂志。Nutr。，46（增刊2），S33-S50。

查看更多我们的淀粉测定试剂盒。

内容：每个试剂盒100个分析

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：抗性淀粉

测定形式：分光光度计

检测方法：吸光度

波长（nm）：510

信号响应：增加

线性范围：每次测定4至100 mg D-葡萄糖

检测限：0.036 g/100 g

反应时间（分钟）：~5小时

应用实例：植物材料、淀粉样品等材料。

方法识别：一种新颖的方法