K-AVCHO_DATA中文版说明书.pdf

Content: 100 assays of each per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Available Carbohydrates, Dietary Fiber

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 340

Signal Response: Increase

Linear Range: 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay

Limit of Detection: 1.475 g/100 g

Reaction Time (min): ~ 5 h

Application examples: Food ingredients, food products and other materials.

Method recognition: AOAC Method 2020.07

The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving 'First Action' status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.

含量：每个试剂盒各100份

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：可用碳水化合物、膳食纤维

测定形式：分光光度计

检测方法：吸光度

波长（nm）：340

信号响应：增加

线性范围：每次测定4至80μg D-葡萄糖、D-果糖或D-半乳糖

检测限：1.475 g/100 g

反应时间（分钟）：~5小时

应用实例：食品配料、食品及其他材料。

方法识别：AOAC方法2020.07

可用碳水化合物测定试剂盒方法适用于测定可用碳水化合物（AVCHO），包括*总可消化淀粉（TDS）加麦芽糊精、蔗糖、D-葡萄糖、D-果糖和乳糖。收到“第一行动”状态的新改进方法：AOAC 2020.07。该方法旨在模拟人体小肠的体内条件（即与PAA+AMG孵育4小时），与膳食纤维（DF）方法的最新进展（K-RINTDF:AOAC方法2017.16）平行，并符合食品法典委员会2009年宣布的DF的新（基于生理学的）定义。此外，蔗糖用一种特定的“蔗糖酶”水解，这种酶（与传统上用于该反应的转化酶不同）对低聚果糖（FOS）没有作用。

*总可消化淀粉（TDS）是指在4小时内被消化的淀粉，是可在人类小肠中消化和吸收的碳水化合物的一部分。