The L-Glutamine/Ammonia (Rapid) test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-glutamine and ammonia in culture media/supernatants and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Content: 50 assays of each (manual) / 500 assays of each (microplate)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Ammonia, L-Glutamine
Assay Format: Spectrophotometer, Microplate
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 1 to 40 µg of L-glutamine per assay
Limit of Detection: 0.54 mg/L (L-glutamine),
0.06 mg/L (ammonia)
Reaction Time (min): ~ 10 min
Application examples: Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.).
Method recognition: Novel method
Advantages
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogense
All enzymes supplied as stabilised suspensions
Only enzymatic kit available
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
L-谷氨酰胺/氨(快速)检测试剂盒是一种专门、方便、经济高效、快速测定和分析培养基/上清液和其他材料中L-谷氨酰胺和氨的新方法。
内容说明:如果所有试剂盒的体积减半,每个试剂盒的手动测试数量可以翻倍。这可以使用MegaQuantTM波分光光度计(D-MQWAVE)轻松调节。
含量:每种50次测定(手动)/500次测定(微孔板)
运输温度:环境温度
储存温度:短期稳定性:2-8oC,
长期稳定性:参见单个组件标签
稳定性:在推荐的储存条件下超过2年
分析物:氨、L-谷氨酰胺
测定形式:分光光度计、微孔板
检测方法:吸光度
波长(nm):340
信号响应:降低
线性范围:每次测定1至40µg L-谷氨酰胺
检测限:0.54mg/L(L-谷氨酰胺),
0.06 mg/L(氨)
反应时间(分钟):~10分钟
应用实例:细胞培养基和培养物、膳食补充剂、蔬菜和其他材料(如生物样品等)。
方法识别:一种新颖的方法
优点
扩展的辅因子稳定性。溶解的辅因子在4摄氏度下稳定1年以上。
由于使用高活性谷氨酰胺酶和不受抑制的谷氨酸脱氢酶,反应非常快
所有酶均以稳定悬浮液的形式提供
仅提供酶试剂盒
非常经济高效
所有试剂在制备后稳定超过2年
Mega Calc™我们的网站上提供了软件工具,可以轻松处理原始数据
包括标准
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