The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Content: 100 assays (50 of each) per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 1 year under recommended storage conditions
Analyte: Ammonia, L-Asparagine, L-Glutamine
Assay Format: Spectrophotometer, Microplate
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 0.2 to 7.0 µg of ammonia, or 0.5 to 50 μg of L-asparagine or L-glutamine per assay
Limit of Detection: 0.50 mg/L (L-asparagine),
0.54 mg/L (L-glutamine),
0.06 mg/L (ammonia)
Reaction Time (min): ~ 20 min
Application examples: Potatoes, potato products, vegetables, cereals and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Novel method
Advantages
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very rapid reaction due to use of uninhibited glutamate dehydrogenase
All enzymes supplied as stabilised suspensions
Only kit available
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
L-天冬酰胺/L-谷氨酰胺/氨检测试剂盒是一种新的方法,用于特定、方便、经济高效和快速地测量和分析食品工业中作为丙烯酰胺前体、细胞培养基/上清液组分或其他材料中的L-天冬胺、L-谷氨酰胺和氨。
内容说明:如果所有试剂盒的体积减半,每个试剂盒的手动测试数量可以翻倍。这可以使用MegaQuantTM波分光光度计(D-MQWAVE)轻松调节。
内容:每个试剂盒100个测定(每个50个)
运输温度:环境温度
储存温度:短期稳定性:2-8oC,
长期稳定性:参见单个组件标签
稳定性:在推荐的储存条件下>1年
分析物:氨、L-天冬氨酸、L-谷氨酰胺
测定形式:分光光度计、微孔板
检测方法:吸光度
波长(nm):340
信号响应:降低
线性范围:每次测定0.2至7.0微克氨,或0.5至50微克L-天冬酰胺或L-谷氨酰胺
检测限:0.50 mg/L(L-天冬酰胺),
0.54mg/L(L-谷氨酰胺),
0.06 mg/L(氨)
反应时间(分钟):~20分钟
应用示例:土豆、土豆制品、蔬菜、谷物和其他材料(如生物培养物、样品等)。
方法识别:一种新颖的方法
优点
扩展的辅因子稳定性。溶解的辅因子在4摄氏度下稳定1年以上。
由于使用了不受抑制的谷氨酸脱氢酶,反应非常迅速
所有酶均以稳定悬浮液的形式提供
仅提供套件
非常经济高效
所有试剂在制备后稳定超过2年
Mega Calc™我们的网站上提供了软件工具,可以轻松处理原始数据
包括标准
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