The K-LACSU kit will allow measurement of lactose in any materials.

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and   therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

The Lactose/Sucrose/D-Glucose Kit should work fine for wort.

Yes, it is possible to specifically measure lactose in a mixture of sucrose, lactose and glucose.  Of course, it is also necessary to measure free glucose (i.e. with no added beta-galactosidase).

The Lactose/Sucrose/D-Glucose Kit will allow measurement of sucrose, lactose and  glucose (not total sugars).  We can supply reagents separately for sucrose or lactose determination (or Glucose).

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme ([email protected]). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
3. State the kit lot number being used (this is found on the outside of the kit box).
4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
6. State the sample type and describe the sample preparation steps if applicable.

The kits we supply are stable at room temperature for several months.  Thus we ship at room temperature.  When you get the kit, it should be stored as recommended and it will be stable for several years.  The kit will be perfectly fine at 30˚C for the shipping time.