K-RINTDF_DATA中文版说明书.pdf

Content: 100 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Dietary Fiber

Assay Format: Enzymatic

Detection Method: Gravimetric/HPLC

Signal Response: Increase

Limit of Detection: 0.5 g/100 g

Total Assay Time: ~ 3 h work (over 1-2 days)

Application examples: Food ingredients, food products and other materials.

Method recognition: AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard Method No. 185 and CODEX Method Type I

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2022.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS4). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced.

In this improved, rapid method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25.

The dietary fiber fractions that are measured with this method are:

1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and High Molecular Weight Soluble Dietary Fiber (SDFP; soluble dietary fiber which is precipitated in the presence of 78% aqueous ethanol), and

2. Low Molecular Weight Soluble Dietary Fiber (SDFS; water soluble dietary fiber that is soluble in the presence of 78% aqueous ethanol).

Alternatively, IDF, SDFP and SDFS can be measured separately.

The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the use of glycerol as an internal standard in the method.

See our full range of dietary fiber assay kits.

View Dietary Fiber Measurement Guide - Which Method for which sample?

* See McCleary, B. V., Sloane, N & Draga, A. (2015). Determination of total dietary fibre and available carbohydrates: a rapid integrated procedure that simulates in vivo digestion. Starch/Starke, 66, 1-24.

Advantages

More rapid measurement - incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed) 

DF values for most samples are very similar to those obtained with AOAC Method 2009.01 

Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01* 

All reagents stable for > 2 years after preparation 

The method is consistent with the CODEX Alimentarius definition of dietary fiber 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Very competitive price (cost per test)

内容：每个试剂盒100个分析

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：膳食纤维

测定形式：酶法

检测方法：重量法/HPLC

信号响应：增加

检测限：0.5 g/100 g

总测定时间：约3小时工作（超过1-2天）

应用实例：食品配料、食品及其他材料。

方法识别：AACC方法32-60.01、AOAC方法2022.01、AOC方法2017.16、ICC标准方法185和CODEX方法I型

快速综合总膳食纤维测定试剂盒方法在合作研究中得到验证（AACC方法32-60.01、AOAC方法2022.01、AOC方法2017.16、ICC标准185），并被食品法典委员会认定为I型方法。K-RINTDF法是测量所有可能含有或不含有抗性淀粉的食物中总膳食纤维的推荐方法。该方法经过更新，更符合人体小肠的体内条件，即4小时的孵育时间。在这些条件下，获得了抗性淀粉的更精确的测量，包括磷酸类交联淀粉（RS4）。使用更高的酶浓度可确保在综合总膳食纤维程序（AOAC方法2009.01和2011.25）的培养条件下，不再产生由非抗性淀粉产生的抗性麦芽糊精。

在这种改进的快速方法中，PAA+AMG的孵育时间减少到4小时，PAA和AMG的水平都增加了，以确保用一组对照样品获得的抗性淀粉水平与回肠造口术数据一致。在这些条件下，大多数样本的DF值与AOAC方法2009.01和2011.25获得的DF值相同。

用这种方法测量的膳食纤维组分为：

1.高分子量膳食纤维（HMWDF），包括不溶性膳食纤维（IDF）和高分子量可溶性膳食纤维（SDFP；在78%乙醇水溶液存在下沉淀的可溶性膳食纤维），以及

2.低分子量可溶性膳食纤维（SDFS；在78%水性乙醇存在下可溶的水溶性膳食纤维）。

或者，IDF、SDFP和SDFS可以单独测量。

该方法中使用的酶纯度高，有效地去除了对其他膳食纤维成分（如β-葡聚糖、果胶和阿拉伯木聚糖）有活性的污染酶。它们以冻干粉的形式供应；允许在该方法中使用甘油作为内标。

查看我们的全套膳食纤维检测试剂盒。

查看膳食纤维测量指南-哪种方法适用于哪个样本？

*参见McCleary，B.V.，Sloane，N&Draga，A.（2015）。总膳食纤维和可用碳水化合物的测定：模拟体内消化的快速综合程序。淀粉/斯塔克，66，1-24。

优点

更快速的测量-与AOAC 2009.01相比，PAA+AMG的孵育时间缩短至4小时（使用的酶水平增加）

大多数样本的DF值与AOAC方法2009.01获得的值非常相似

快速综合总膳食纤维法消除了AOAC方法2009.01确定的所有限制*

所有试剂在制备后稳定超过2年

该方法符合食品法典委员会对膳食纤维的定义

Mega Calc™ 我们的网站上提供了软件工具，可以轻松处理原始数据

极具竞争力的价格（每次测试的成本）