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To choose a chapter, play the video and select the required chapter from the options on the video display.
Chapter 1: Kit Details
Chapter 2: Principle of the Assay
Chapter 3: Kit Contents
Chapter 4: Reagent Preparation
Chapter 5: Weighing of Samples, Addition of Buffer plus Amylase & AMG
Chapter 6: Addition of Tris Base and Inactivation of Amylase & AMG
Chapter 7: Incubation with Protease
Chapter 8A: Determination of HMWDF & LMWSDF
(i): pH Adjustment & Addition of D-Sorbitol & Ethanol
(ii): Filtration & Recovery of HMWDF & LMWSDF
(iii): Ash & Protein Determination
(iv): Evaporation of Aqueous Ethanol & Recovery of LMWSDF
(vi): Desalting of Samples with Ion Exchange Resin
(vii): Liquid Chromatography of Desalted Samples
Chapter 8B: Determination of IDF, HMWDF & LMWSDF
(i): pH Adjustment of the Incubation Mixture and Addition of D-Sorbitol
(ii): Filtration and Recovery of IDF, HMWDF & LMWSDF
Chapter 9: Calculation of Dietary Fiber Content
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