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总膳食纤维快速整合试剂盒Rapid Integrated Total Dietary Fibre(K-RINTDF)视频

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Chapter 1: Principle

Chapter 2: Kit Contents

Chapter 3: Reagent Preparation

Chapter 4: Weighing of Samples, Addition of Buffer plus Amylase & AMG 

                and Incubation at 37ºC for 16 hours 

Chapter 5: Addition of Tris Base and Inactivation of Amylase & AMG

Chapter 6: Incubation with Protease

Chapter 7A: Determination of HMWDF & LMWSDF

                   pH Adjustment & Addition of Glycerol & Ethanol

              (i): Filtration & Recovery of HMWDF & LMWSDF

             (ii):  Ash Determination

             (iii): Protein Determination

             (iv): Evaporation of Aqueous Ethanol & Recovery of LMWSDF

              (v): Desalting of Samples with Ion Exchange Resin

             (vi):  Liquid Chromatography of Desalted Samples

Chapter 7B: Determination of IDF, HMWSDF & LMWSDF           

              (i): Filtration and Recovery of IDF, HMWSDF & LMWSDF

Chapter 8: Calculation of Dietary Fibre Content

相关视频:

Rapid Integrated Total Dietary Fiber Assay Kit (K-RINTDF)

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