K-DSTRS_DATA中文版说明书.pdf

Content: 40 assays of each per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Digestible Starch, Resistant Starch, Total Starch

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 4 to 100 μg of D-glucose per assay

Limit of Detection: 3.1 g/100 g

Reaction Time (min): ~ 360 min

Application examples: Plant materials, starch samples and other materials.

The Digestible and Resistant Starch Assay Kit (K-DSTRS) for the determination of digestible, resistant and total starch in starch samples, plant and other materials.

This method is based on the research of Englyst et al. (Ref) with some modifications. Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal.

In line with Englyst definitions:

Rapidly digestible starch (RDS) is that starch which is digested within 20 min.

Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.

A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).

Resistant starch (RS) then, is that starch which is not digested within 4 h.

The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009.

See our full range of starch and dietary fiber assay kits.

含量：每个试剂盒各40份

运输温度：环境温度

储存温度：短期稳定性：2-8oC，

长期稳定性：参见单个组件标签

稳定性：在推荐的储存条件下超过2年

分析物：可消化淀粉、抗性淀粉、总淀粉

测定形式：分光光度计

检测方法：吸光度

波长（nm）：510

信号响应：增加

线性范围：每次测定4至100μg D-葡萄糖

检测限：3.1 g/100 g

反应时间（分钟）：~360分钟

应用实例：植物材料、淀粉样品等材料。

可消化和抗性淀粉测定试剂盒（K-DSTRS），用于测定淀粉样品、植物和其他材料中的可消化、抗性和总淀粉。

该方法基于Englyst等人的研究。（参考文献）进行了一些修改。消化是使用饱和水平的胰腺α-淀粉酶（PAA）和淀粉葡萄糖苷酶（AMG）进行的，但在搅拌容器而不是摇动管中进行，以简化样品去除。

根据Englyst的定义：

快速消化淀粉（RDS）是指在20分钟内消化的淀粉。

慢消化淀粉（SDS）是指在20到120分钟之间被消化的淀粉。

引入了一个新术语“总可消化淀粉（TDS）”（并对其进行了测量），以涵盖4小时内（食物在人体小肠中的平均停留时间）消化的所有淀粉。

抗性淀粉（RS）是指在4h内未被消化的淀粉。

培养条件与AOAC方法2017.16中使用的条件相似，AOAC方法是一种新的快速综合的总膳食纤维测量方法（Megazyme方法K-RINTDF）。该方法以生理学为基础，旨在符合食品法典委员会2009年宣布的DF定义。

查看我们的全套淀粉和膳食纤维检测试剂盒。