The D-Xylose test kit is a novel method for the specific, convenient and rapid measurement and analysis of D-xylose in plant extracts, culture media/supernatants and other materials.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of D-Xylose in fermentation
broths and hydrolysates of plant material and polysaccharides
Principle:
(xylose mutarotase)
(1) α-D-Xylose ↔ β-D-xylose
(β-xylose dehydrogenase)
(2) β-D-Xylose + NAD+ → D-xylonic acid + NADH + H+
Kit size: 100 assays (manual) / 1000 (microplate)
/ 1300 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 6 min
Detection limit: 0.7 mg/L
Application examples:
Analysis of D-xylose in fermentation broths and hydrolysates of plant
material and polysaccharides
Method recognition: Novel method
Advantages
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
Yes. K-XYLOSE can be used to measure D-xylose in urine samples assuming that the D-xylose concentration is above the limit of detection for the kit assay after sample preparation. An example sample preparation method for biological samples is provided in the K-XYLOSE data booklet.
K-XYLOSE is able to specifically measure xylose in samples that contain up to 5 mg of glucose in the final assay reaction without any interference by the glucose.
The kit assay will only measure the non-covalently linked monosaccharide.
Oligosaccharides or polysaccharides can be measured after hydrolysis to the monosaccharide. Generally acid hydrolysis can be achieved by boiling the oligo/polysaccharide in 1.3 M HCl for 1 h. It is recommended that scientific literature is consulted for information on hydrolysis conditions for the particular oligo/polysaccharide that is being measured.
Yes, instead of adding 2 μL of enzyme suspension an alternative is to dilute the enzyme and add a larger volume to the microplate assay.
Dilute the assay buffer 10-fold with distilled water and use this as the diluent to dilute an aliquot of the enzyme suspension also by 10-fold. Instead of 2 μL, use 20 μL of the diluted enzyme in the microplate assay.
Megazyme D-木糖检测试剂盒操作视频(K-XYLOSE)
Megazyme 溶解淀粉 操作视频
Megazyme 试剂盒样品前处理准备操作视频
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