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密三糖或棉子糖/D-半乳糖检测试剂盒
密三糖或棉子糖/D-半乳糖检测试剂盒

英文名:Raffinose/D-Galactose Assay Kit

货号:K-RAFGA

规格:120 assays per kit

市场价: 4982
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K-RAFGA_DATA中文版说明书.pdf

 

The Raffinose/D-Galactose test kit allows for the specific and rapid measurement of raffinose and D-galactose in plant materials and food products.

 

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

 

View all of our monosaccharide test kit products.

 

Content: 120 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: D-Galactose, Raffinose

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 340

Signal Response: Increase

Linear Range: 4 to 83 µg of D-galactose per assay (i.e. approx. 12 to 250 µg of raffinose per assay)

Limit of Detection: 21 mg/L

Reaction Time (min): ~ 60 min

Application examples: Cereal flours, soybean flour, by-products of sucrose manufacture and other materials.

Method recognition: Used and accepted in food analysis

 

Advantages

Very rapid reaction due to inclusion of galactose mutarotase (patented technology) 

Very competitive price (cost per test) 

All reagents stable for > 2 years after preparation 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Standard included

 

棉子糖/D-半乳糖检测试剂盒可用于植物材料和食品中棉子糖和D-半乳糖的特异性快速测量。

内容说明:如果所有试剂盒的体积减半,每个试剂盒的手动测试数量可以翻倍。这可以使用MegaQuantTM波分光光度计(D-MQWAVE)轻松调节。

查看我们所有的单糖检测试剂盒产品。

内容:每个试剂盒120次检测

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:D-半乳糖、拉菲糖

测定形式:分光光度计

检测方法:吸光度

波长(nm):340

信号响应:增加

线性范围:每次测定4至83µg D-半乳糖(即每次测定约12至250µg棉子糖)

检测限:21 mg/L

反应时间(分钟):~60分钟

应用实例:谷物粉、大豆粉、蔗糖生产副产品等原料。

方法识别:在食品分析中使用和接受

优点

由于含有半乳糖变位酶,反应非常迅速(专利技术)

极具竞争力的价格(每次测试的成本)

所有试剂在制备后稳定超过2年

Mega Calc™我们的网站上提供了软件工具,可以轻松处理原始数据

包括标准

 

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. Sometimes a negative absorbance change is obtained for the blank samples, is this normal? Should the real value (negative absorbance change) or “0” be used in the calculation of results?

Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.

Q3. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.
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密三糖或棉子糖/D-半乳糖检测试剂盒
密三糖或棉子糖/D-半乳糖检测试剂盒

120 assays per kit

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