分析物意义: 快速消化和吸收的糖及膳食纤维
Megazyme检测试剂盒优点:检测程序新颖、试剂稳定
An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products. Content:100 assays of each component
An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products
Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H2O → glucose
(protease)
(2) Protein + H2O → peptides
(3) Dietary fiber determined gravimetrically following
alcohol precipitation
Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose
(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP+ → gluconate-6-phosphate
+ NADPH + ADP + H+
Kit size: 100 assays of each
Application examples:
Food ingredients, food products and other materials
Method recognition:
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)
Advantages
Q1. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
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