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Megazyme Beta葡聚糖[混联]检测试剂盒 K-BGLU

英文名:β-Glucan Assay Kit (Mixed Linkage)

货号:K-BGLU

规格:100 assays per kit

市场价: 4719
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K-BGLU 中文版说明书下载

 

Content: 100 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: β-Glucan

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 4 to 100 μg of D-glucose per assay

Limit of Detection: 0.5 g/100 g

Total Assay Time: ~ 100 min

Application examples: Oats, barley, malt, wort, beer, food and other materials.

Method recognition: AACC Method 32-23.01, AOAC Method 995.16, AOAC Method 992.28, CODEX Method Type II, EBC Method 3.10.1, ICC Standard No. 166 and RACI Standard Method

 

The Beta-Glucan test kit is suitable for the measurement and analysis of Beta-Glucan (Mixed Linkage).

For the measurement of 1,3:1,4-β-D-glucan in cereal grains, milling fractions, wort, beer and other food products.

See our complete range of polysaccharide assay kits.

 

内容:每个试剂盒100个分析

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:β-葡聚糖

测定形式:分光光度计

检测方法:吸光度

波长(nm):510

信号响应:增加

线性范围:每次测定4至100μg D-葡萄糖

检测限:0.5 g/100 g

总测定时间:~100分钟

应用实例:燕麦、大麦、麦芽、麦芽汁、啤酒、食品等原料。

方法识别:AACC方法32-23.01、AOAC方法995.16、AOAC法992.28、CODEX方法类型II、EBC方法3.10.1、ICC标准166和RACI标准方法

β-葡聚糖检测试剂盒适用于β-葡聚糖(混合连锁)的测量和分析。

用于测量谷物、碾磨部分、麦芽汁、啤酒和其他食品中的1,3:1,4-β-D-葡聚糖。

查看我们的全套多糖检测试剂盒。

 

 

 

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q4. I have purchased barley beta-glucan (lot 30108) and carob galactomannan low viscosity (lot 30702) and would like to know what else there might be in these substrates.

Barley beta-glucan lot 30108 would contain about 3-4% arabinoxylan (this was produced in 1993).  Material supplied post 1995 contains < 0.5% arabinoxylan.  Carob galactomannan is quite pure (> 96%).

Q5. Although not specified on the beta-glucan data sheet, is the ratio of 1-3 to 1-4 bonds measured? If so, what is the ratio and would you expect it to remain standardised over a number of different batches?

The content of 1,3 bonds in barley beta-glucan is about 32% (from literature).  We would not expect this to change much (if at all) over different batches.

Q6. Both substrates; barley beta-glucan and wheat arabinoxylan, are standardised to a specific viscosity, e.g. 23-24 cSt. Are the substrates adjusted to give this viscosity?

The substrates are enzymically treated to yield the required viscosity (20 ~ 30 cSt). Modification of the viscosity of beta-glucan is required for IOB viscometric malt beta-glucanase test (which works well).

Q7. We are setting up reducing-sugar assays for both beta-glucanase and xylanase from different fungal origins. Which barley beta-glucan do you recommend for the glucanase assay.

For glucanase assay we recommend the medium viscosity beta-glucan.  We now offer low, medium and high viscosity wheat arabinoxylans, and we think that the low viscosity material will be best (easiest) to use in the reducing-sugar assay.

Equl Megazyme Beta葡聚糖[混联]检测试剂盒 β-Glucan Assay Kit (Mixed Linkage) K-BGLU

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Megazyme Beta葡聚糖[混联]检测试剂盒 K-BGLU
Megazyme Beta葡聚糖[混联]检测试剂盒 K-BGLU

100 assays per kit

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