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分析物意义:常见食品组分,或作为甜味剂,或用于改善口感
Megazyme检测试剂盒优点:新型的药片模式,性质更稳定,反应快
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials
Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate
(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+
Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK
Advantages