分析物意义：常见的天然食品组分，例如在奶酪和番茄中或调味剂中，如味精 

Megazyme检测试剂盒优点：提供的硫辛酰胺脱氢酶是稳定的悬浮液而不是可溶性粉末，从而减少了酶的浪费 

The L-Glutamic Acid test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of L-glutamate (MSG) in foodstuffs.
Suitable for manual, auto-analyser and microplate formats.

Colourimetric method for the determination of L-Glutamic Acid
(Monosodium Glutamate; MSG) in foodstuffs and other materials

Principle:
(beef liver glutamate dehydrogenase)
(1) L-Glutamic acid + NAD⁺ + H₂O ↔ 2-oxoglutarate + NADH + NH₄⁺

(diaphorase)
(2) INT + NADH + H⁺ → NAD⁺ + INT-formazan

Kit size:                            60 assays (manual) / 600 (microplate) 
                                         / 700 (auto-analyser)
Method:                            Spectrophotometric at 492 nm
Reaction time:                  ~ 9 min
Detection limit:                 0.21 mg/L
Application examples: 
Fruit and vegetables (e.g. tomato), processed fruit and vegetables
(e.g. tomato puree / juice, ketchup, soy sauce), condiments, processed
meat products (e.g. extracts, bouillon and sausages), soup, pharmaceuticals
and other materials (e.g. biological cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by ISO, GOST
and NMKL

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 2 years after preparation
  
• Glutamate dehydrogenase solution stable at -20°C
  
• No wasted diaphorase solution (stable suspension supplied)
  
• Rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats