柠檬酸检测试剂盒

货号:K-CITR
英文名:Citric Acid Assay Kit
规格: 72 assays (manual) / 720 assays
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K-CITR_DATA中文版说明书.pdf

 

The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

Content: 72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Citric Acid

Assay Format: Spectrophotometer, Microplate, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 340

Signal Response: Decrease

Linear Range: 1.0 to 100 µg of citric acid per assay

Limit of Detection: 0.491 mg/L

Reaction Time (min): ~ 5 min

Application examples: Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products (e.g. cheese), meat, processed meat, vegetable and fruit products, bakery products, paper, pharmaceuticals, cosmetics and other materials (e.g. biological cultures, samples, etc.).

Method recognition: Methods based on this principle have been accepted by MEBAK, OIV, EU, ISO2963, AOAC and IFU22 (NOTE: If the enzyme oxaloacetate decarboxylase is present in the sample, some of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed to efficiently convert any pyruvate produced into D-lactate and NAD+).

 

Advantages

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Reconstituted citrate lyase stable for 4 weeks at 4oC / 6 months below -10oC 

Buffer / cofactor / enzyme tablets for efficient use of kit components 

PVP incorporated to prevent tannin inhibition 

Very competitive price (cost per test) 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Standard included

Suitable for manual, microplate and auto-analyser formats

 

柠檬酸检测试剂盒是一种灵活简单的方法,用于快速可靠地测量和分析食品、饮料和其他材料中的柠檬酸。

内容说明:如果所有试剂盒的体积减半,每个试剂盒的手动测试数量可以翻倍。这可以使用MegaQuantTM波分光光度计(D-MQWAVE)轻松调节。

内容:72次测定(手动)/720次测定(微孔板)/840次测定(自动分析仪)

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:柠檬酸

测定形式:分光光度计、微孔板、自动分析仪

检测方法:吸光度

波长(nm):340

信号响应:降低

线性范围:每次测定1.0至100µg柠檬酸

检出限:0.491 mg/L

反应时间(分钟):~5分钟

应用示例:葡萄汁、葡萄酒、啤酒、果汁、软饮料、茶、乳制品(如奶酪)、肉类、加工肉类、蔬菜和水果产品、烘焙产品、纸张、药品、化妆品和其他材料(如生物培养物、样品等)。

方法识别:基于该原理的方法已被MEBAK、OIV、EU、ISO2963、,AOAC和IFU22(注:如果样品中存在草酰乙酸脱羧酶,部分草酰乙酸产物会转化为丙酮酸。因此,为了确保柠檬酸的定量测量,使用D-乳酸脱氢酶(D-LDH)将产生的丙酮酸有效转化为D-乳酸和NAD+)。

优点

扩展的辅因子稳定性。溶解的辅因子在4摄氏度下稳定1年以上。

重建的柠檬酸裂解酶在低于-10摄氏度的4摄氏度下稳定4周/6个月

高效使用试剂盒成分的缓冲液/辅因子/酶片

加入PVP以防止单宁抑制

极具竞争力的价格(每次测试的成本)

Mega Calc™我们的网站上提供了软件工具,可以轻松处理原始数据

包括标准

适用于手动、微板和自动分析仪格式