分析物意义：  快速消化和吸收的糖及膳食纤维

Megazyme检测试剂盒优点：检测程序新颖、试剂稳定 

An integrated procedure for the measurement of available carbohydrates and dietary fibre in cereal products, fruit and vegetables and food products. Content:100 assays of each component

An integrated procedure for the measurement of Available
Carbohydrates and Dietary Fiber in cereal products, fruit and
vegetables and food products

Principle (Dietary Fiber):
(α-amylase + amyloglucosidase)
(1) Starch + H₂O → glucose

(protease)
(2) Protein + H₂O → peptides

(3) Dietary fiber determined gravimetrically following
alcohol precipitation

Principle (Available Carbohydrates):
(sucrase / maltase + β-galactosidase)
(4) Sucrose, maltose and lactose → D-glucose + D-fructose
+ D-galactose

(PGI, hexokinase + glucose-6-phosphate dehydrogenase)
(5) D-Glucose + D-fructose + ATP + NADP⁺ → gluconate-6-phosphate
+ NADPH + ADP + H⁺

Kit size:                             100 assays of each
Application examples: 
Food ingredients, food products and other materials
Method recognition:     
Dietary Fibre - AOAC (Methods 985.29, 991.42, 991.43 and 993.19)
and AACC (Methods 32-05.01, 32-07.01 and 32-21.01)

Advantages

• Very cost effective
  
• All reagents stable for > 2 years after preparation
  
• High purity / standardised enzymes employed
  
• Only kit available
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Simple format