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Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名: β-Glucan Assay Kit (Yeast & Mushroom)

货号:K-YBGL

规格:100 assays per kit

市场价: 4719
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K-YBGL 中文版说明书

Content: 100 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: β-Glucan

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 4 to 100 μg of glucose per assay

Limit of Detection: 1 g/100 g

Total Assay Time: ~ 100 min

Application examples: Yeast preparations, mushroom preparations and other materials.

Method recognition: Novel method

Advantages

Very cost effective

All reagents stable for > 12 months after preparation 

Only enzymatic kit available 

Simple format 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Standard included

The β-Glucan Assay Kit (Yeast and Mushroom) is suitable for the indirect measurement of 1,3:1,6-β-glucan in yeast and mushroom preparations containing starch, glycogen, sucrose and trehalose.

 

We are happy to announce a new and improved procedure!

 

Trehalase enzyme has now been added to the β-Glucan Assay Kit (Yeast and Mushroom), further increasing the accuracy of β-glucan measurement for certain samples. The addition of trehalase removes instances of overestimation of β-glucan content in samples containing trehalose. Please refer to the assay protocol and the FAQ here for more information.

 

See our complete list of available polysaccharide assay kits.

 

内容:每个试剂盒100个分析

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:β-葡聚糖

测定形式:分光光度计

检测方法:吸光度

波长(nm):510

信号响应:增加

线性范围:每次测定4至100μg葡萄糖

检测限:1 g/100 g

总测定时间:~100分钟

应用实例:酵母制剂、蘑菇制剂等原料。

方法识别:一种新颖的方法

优点

极具成本效益

所有试剂在制备后可稳定12个月以上

只有酶试剂盒可用

简单格式

Mega Calc™ 我们的网站上提供了软件工具,可以轻松处理原始数据

包含标准

β-葡聚糖测定试剂盒(酵母和蘑菇)适用于间接测定含有淀粉、糖原、蔗糖和海藻糖的酵母和蘑菇制剂中的1,3:1,6-β-葡聚糖。

我们很高兴宣布一个新的和改进的程序!

Trehalase酶现已添加到β-葡聚糖检测试剂盒(酵母和蘑菇)中,进一步提高了某些样品β-葡聚糖测量的准确性。添加海藻糖消除了含有海藻糖的样品中β-葡聚糖含量过高的情况。有关更多信息,请参阅此处的化验协议和常见问题解答。

查看我们可用的多糖检测试剂盒的完整列表。

 

 

 

Q1. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample,  in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

 

Q2. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Megazyme 酵母菌β-葡聚糖酶法检测试剂盒操作视频(K-YBGL)

Megazyme 溶解淀粉 操作视频

Megazyme 试剂盒样品前处理准备操作视频

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Beta葡聚糖[酵母和蘑菇]检测试剂盒
Beta葡聚糖[酵母和蘑菇]检测试剂盒

100 assays per kit

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