The Ascorbic Acid (L-Ascorbate) assay kit is for the specific measurement and analysis of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Content: 40 assays (manual) / 400 assays (microplate) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Ascorbic Acid
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 578
Signal Response: Increase
Linear Range: 0.5 to 30 µg of L-ascorbic acid per assay
Limit of Detection: 0.175 mg/L
Reaction Time (min): ~ 8 min
Application examples: Wine, beer, fruit juices, soft drinks, jam, milk, dairy products (e.g. cheese), dietetic foods, baby foods, processed meat, baking additives, fruit and vegetables (e.g. tomato and potato), pharmaceuticals, feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by MEBAK
Advantages
Very competitive price (cost per test)
All reagents stable for > 6 months after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
抗坏血酸（L-抗坏血酸）测定试剂盒用于饮料、肉类、面粉、乳制品和蔬菜产品中L-抗坏血酸的特定测量和分析。
内容说明：如果所有试剂盒的体积减半，每个试剂盒的手动测试数量可以翻倍。这可以使用MegaQuantTM波分光光度计（D-MQWAVE）轻松调节。
内容：40次测定（手动）/400次测定（微孔板）/400个测定（自动分析仪）
运输温度：环境温度
储存温度：短期稳定性：2-8oC，
长期稳定性：参见单个组件标签
稳定性：在推荐的储存条件下超过2年
分析物：抗坏血酸
测定形式：分光光度计、微孔板、自动分析仪
检测方法：吸光度
波长（nm）：578
信号响应：增加
线性范围：每次测定0.5至30µg L-抗坏血酸
检测限：0.175 mg/L
反应时间（分钟）：~8分钟
应用示例：葡萄酒、啤酒、果汁、软饮料、果酱、牛奶、乳制品（如奶酪）、减肥食品、婴儿食品、加工肉、烘焙添加剂、水果和蔬菜（如番茄和土豆）、药品、饲料和其他材料（如生物培养物、样品等）。
方法识别：基于此原理的方法已被MEBAK接受
优点
极具竞争力的价格（每次测试的成本）
所有试剂在制备后稳定>6个月
Mega Calc™我们的网站上提供了软件工具，可以轻松处理原始数据
包括标准
适用于手动、微板和自动分析仪格式
Yes. If the assay format is not available for your auto-analyser you can supply the following parameters of your relevant auto-analyser to Megazyme and an appropriate assay format may be available:
Parameters:
Instrument make and model:
Light-path length:
Reagent addition volumes (e.g. R1 volume, R2 volume, R3 if applicable):
Suggested auto-analyser format for K-ASCO:
Prepare the reagents as described in the K-ASCO data booklet then prepare R1 and R2 as follows:
Preparation of R1: (90 assays)
|
Component |
Volume |
|
Bottle 1 |
5.6 mL |
|
Bottle 4 |
0.225 mL |
|
Distilled water |
17.5 mL |
|
Total volume |
23.325 mL |
Preparation of R2: (90 assays)
|
Component |
Volume |
|
Bottle 2 |
2.25 mL |
|
Bottle 3 |
2.25 mL |
|
Total volume |
4.5 mL |
EXAMPLE METHOD:
R1: 0.260 mL
Sample: ~ 0.005 mL
R2: 0.05 mL
Note: For accurate measurement of ascorbic acid each sample requires two measurements, one with AAO (bottle 4) present and one with AAO (bottle 4) absent.
In the auto-analyser format described above there is no option to read A1 as in the cuvette assay. In this instance use an ascorbic acid calibration curve to calculate results. Alternatively add 0.025 mL of bottle 2 and 3 separately to mimic the cuvette assay and allow the reading A1 (this will require 3 reagent additions) OR it may be possible to add the MTT (bottle 2) with R1 as follows:
Preparation of R1: (90 assays)
|
Component |
Volume |
|
Bottle 1 |
5.6 mL |
|
Bottle 2 |
2.25 mL |
|
Bottle 4 |
0.225 mL |
|
Distilled water |
17.5 mL |
|
Total volume |
25.575 mL |
Preparation of R2: (90 assays)
|
Component |
Volume |
|
Bottle 2 |
2.25 mL |
|
Bottle 3 |
2.25 mL |
|
Total volume |
4.5 mL |
EXAMPLE METHOD:
R1: 0.285 mL
Sample: ~ 0.005 mL
R2: 0.025 mL
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Sometimes the addition of the last assay component can cause a small negative absorbance change in the blank samples due to a dilution effect and in such cases it is recommended that the real absorbance values be used in the calculation of results.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
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