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蔗糖/D-葡萄糖检测试剂盒
蔗糖/D-葡萄糖检测试剂盒

英文名:Sucrose/D-Glucose Assay Kit

货号:K-SUCGL

规格:250 assays per kit

市场价: 4240
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K-SUCGL_DATA中文版说明书.pdf

The Sucrose/D-Glucose assay kit is suitable for the measurement of sucrose and D-glucose in fruit juice, beverages, honey and food products.

 

The Megazyme Sucrose/D-Glucose Test Kit employs high purity glucose oxidase, peroxidase and β-fructosidase (invertase) and can be used with confidence for the specific measurement of D-glucose and sucrose in plant and food extracts.

 

Browse all of our monosaccharide and disaccharide assay kits.

 

Content: 250 assays per kit

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: D-Glucose, Sucrose

Assay Format: Spectrophotometer

Detection Method: Absorbance

Wavelength (nm): 510

Signal Response: Increase

Linear Range: 10 to 100 μg of D-glucose per assay

Limit of Detection: 100 mg/L

Reaction Time (min): ~ 30 min

Application examples: Beer, fruit juices, soft drinks, coffee, milk, jam, honey, dietetic foods, bread, bakery products, candies, chocolate, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.).

Method recognition: Used and accepted in food analysis

 

Advantages

Very competitive price (cost per test) 

All reagents stable for > 12 months after preparation 

Simple format 

Mega-Calc™ software tool is available from our website for hassle-free raw data processing 

Standard included

 

蔗糖/D-葡萄糖测定试剂盒适用于果汁、饮料、蜂蜜和食品中蔗糖和D-葡萄糖的测定。

Megazyme蔗糖/D-葡萄糖检测试剂盒采用高纯度葡萄糖氧化酶、过氧化物酶和β-果糖苷酶(转化酶),可用于植物和食品提取物中D-葡萄糖和蔗糖的特异性测量。

浏览我们所有的单糖和双糖检测试剂盒。

内容:每个试剂盒250个分析

运输温度:环境温度

储存温度:短期稳定性:2-8oC,

长期稳定性:参见单个组件标签

稳定性:在推荐的储存条件下超过2年

分析物:D-葡萄糖、蔗糖

测定形式:分光光度计

检测方法:吸光度

波长(nm):510

信号响应:增加

线性范围:每次测定10至100μg D-葡萄糖

检测限:100 mg/L

反应时间(分钟):~30分钟

应用示例:啤酒、果汁、软饮料、咖啡、牛奶、果酱、蜂蜜、减肥食品、面包、烘焙产品、糖果、巧克力、甜点、糖果、冰淇淋、水果和蔬菜、调味品、烟草、化妆品、药品、纸张和其他材料(如生物培养物、样品等)。

方法识别:在食品分析中使用和接受

优点

极具竞争力的价格(每次测试的成本)

所有试剂在制备后可稳定12个月以上

简单格式

Mega Calc™我们的网站上提供了软件工具,可以轻松处理原始数据

包括标准

 Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme (cs@megazyme.com). Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q3. How can I work out how much sample to extract and what dilution of my sample should be used in the kit assay?

Where the amount of analyte in a liquid sample is unknown, it is recommended that a range of sample dilutions are prepared with the aim of obtaining an absorbance change in the assay that is within the linear range.
Where solid samples are analysed, the weight of sample per volume of water used for sample extraction/preparation can be altered to suit, as can the dilution of the extracted sample prior to the addition of the assay, as per liquid samples.

Q4. The pH of my sample is low (pH ~ 3.0), do I need to adjust this before I use the sample in the kit assay?

The final pH of the kit assay after the sample is added should not change from what it should be (as stated in the kit for the assay buffer). If it does change then the sample will require pH adjustment. In most cases the sample volume being used is low relative to the final assay volume and in this case the pH of the kit assay is unlikely to be affected.

Q5. Can you explain, step by step, how to follow the method and perform the kit assay?

For users who are not familiar with how to use the Megazyme tests kits then it is recommended that they follow this example, e.g. D-Fructose/D-Glucose Assay kit K-FRUGL (http://secure.megazyme.com/D-Fructose-D-Glucose-Assay-Kit):

1. The kit components are listed on pages 2-3 of the kit booklet.
2. Prepare the kit reagents as described on page 3.
3. For separate measurements of glucose and fructose follow procedure A on page 4.
4. Pipette the volumes listed for water, sample, solution 1 and solution 2 into 3 mL, 1 cm pathlength cuvettes. Duplicate sample assays and duplicate blanks are recommended. Mix the contents of each cuvette by inversion (seal the cuvette using parafilm or a plastic cuvette cap – do not use a finger) then after ~3 min record the first absorbance reading of each cuvette at 340 nm (this is reading A1).
5. Then add suspension 3 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then record the absorbance reading of each cuvette at 340 nm (this is reading A2). NB. It is essential that the reaction is compete. To assess this, record the absorbances at ~ 2 minute intervals and until the absorbance plateaus. A stable absorbance indicates that the reaction is complete. If the absorbance continues to increase then continue to record absorbances until it plateaus and only then record absorbance reading A2.
6. Then add suspension 4 and mix the contents of each cuvette by inversion. Incubate for 5 minutes then take absorbance reading of each cuvette at 340 nm (this is reading A3). NB. As above, assess that the reaction has completed by take subsequent readings at ~2 min intervals.
7. For simple, automated results analysis, input the absorbance readings (A1, A2, A3) for samples and blanks into the K-FRUGL MegaCalc.

To ensure that the assay is working, and being performed correctly it is recommend that the test is performed using the standard sample that is provided with the kit and to obtain the expected values before proceeding to test real samples.
It is recommend that new users also watch this video which highlights how to perform the assays.
Many of the other Megazyme test kits follow a similar format.

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蔗糖/D-葡萄糖检测试剂盒
蔗糖/D-葡萄糖检测试剂盒

250 assays per kit

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